Application of vacuum collector

The blood collection vessels are distinguished according to the color of the cap.

1. The common serum tube has a red cap, and the blood collection vessel does not contain additives. It is used for routine serum biochemistry, blood bank and serological related tests.

2. The rapid serum tube has a red cap, and there is a coagulant in the blood collection vessel to accelerate the coagulation process. The rapid serum tube can coagulate the collected blood within 8 minutes, which is suitable for emergency serum serialization test.

3. The separating gel and coagulant are added into the collecting vessel with a yellow head of the separating gel and coagulant. After the specimen is centrifuged, the separating gel can completely separate the liquid components (serum or plasma) and solid components (red blood cells, white blood cells, platelets, fibrin, etc.) in the blood and completely accumulate in the center of the test tube to form a barrier. The specimen remains stable within 48 hours. Coagulant can quickly activate the coagulation mechanism and accelerate the coagulation process. It is suitable for emergency serum biochemical test.

4. The green cap of heparin anticoagulant tube is filled with heparin in the blood collection vessel. Heparin has the function of antithrombin directly, which can prolong the coagulation time of samples. It is suitable for erythrocyte fragility test, blood gas analysis, hematocrit test, ESR and universal biochemical determination, but not for hemagglutination test. Excessive heparin can cause leukocyte aggregation and cannot be used for leukocyte counting.

5. The purple cap of EDTA anticoagulant tube, ethylenediamine tetraacetic acid (EDTA, molecular weight 292) and its salt are amino polycarboxylic acids, which can effectively chelate calcium ions in blood samples, chelate calcium or remove calcium reaction sites, block and terminate endogenous or exogenous coagulation process, so as to prevent blood samples from coagulation. It is suitable for general hematology test, not for coagulation test and platelet function test, nor for the determination of calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and leucine aminopeptidase, and suitable for PCR test.

6. The blue cap of sodium citrate coagulation test tube. Sodium citrate mainly plays an anticoagulant role by chelating with calcium ions in blood samples. It is applicable to coagulation experiments. The anticoagulant concentration recommended by the National Committee for clinical laboratory standards (NCCLS) is 3.2% or 3.8% (equivalent to 0.109mol/l or 0.129mol/l), and the ratio of anticoagulant to blood is 1:9.

7. The black cap of sodium citrate ESR test tube. The concentration of sodium citrate required by ESR test is 3.8% (equivalent to 0.109mol / L). The ratio of anticoagulant to blood is 1:4.

8. Potassium oxalate / sodium fluoride gray cap. Sodium fluoride is a weak anticoagulant. It is usually used in combination with potassium oxalate or sodium ethyliodate. The proportion is 1 part of sodium fluoride and 3 parts of potassium oxalate. 4mg of this mixture can make 1ml blood not coagulate and inhibit sugar decomposition within 23 days. It is a good preservation agent for blood glucose determination. It can not be used for urea determination by Urease method, nor for alkaline phosphatase and amylase determination. It is recommended for blood glucose detection.

The above is all about the use of vacuum blood collection vessels introduced by Xiaobian. If you are still interested in relevant contents, please continue to pay attention to Cangzhou Fukang Pharmaceutical Products Co., Ltd